True

HT Medium Suplement 50X, Liquid w/ 680.5 mg per litre Hypoxanthine and 193.8 mg/litre Thymidine in Phosphate Buffered Saline 10ml HT supplement is sufficient for 500ml medium

[TCL073] The specific action of aminopterin present in the HAT system is to inhibit the de novo DNA synthesis. Hence, it needs to be eliminated once the hybrids are selected and isolated. Aminopterin is diluted by several passages in hypoxanthine-thymidine (HT) supplemented medium for about 2-3 weeks. Once the de novo nucleoside-synthesis pathway has been reinstated, HT supplementation can be discontinued.
TCL073 is a sterile filtered, cell culture tested 50X HT medium supplement containing 680.5 mg/litre Hypoxanthine and 193.8 mg/litre Thymidine in Phosphate Buffered Saline.

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[TCL073] The specific action of aminopterin present in the HAT system is to inhibit the de novo DNA synthesis. Hence, it needs to be eliminated once the hybrids are selected and isolated. Aminopterin is diluted by several passages in hypoxanthine-thymidine (HT) supplemented medium for about 2-3 weeks. Once the de novo nucleoside-synthesis pathway has been reinstated, HT supplementation can be discontinued. TCL073 is a sterile filtered, cell culture tested 50X HT medium supplement containing 680.5 mg/litre Hypoxanthine and 193.8 mg/litre Thymidine in Phosphate Buffered Saline.

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